Genetic Characterization of Mangosteen (Garcinia mangostana L.) Using SSR Markers

Abstract

Assessment and utilization of diversity in plant genetic resources is very important to improve of plant species. A 
mangosteen (Garcinia mangostana L.) seed was formed obligate apomicts process. Genetic diversity of mangosteen 
was developed by using SSR markers. An inter-simple sequence repeat (ISSR)-suppression - PCR technique established 
to SSR marker of plant spesies. DNA library construction was used restriction enzyme blunt end Rsa I and adaptor 
(consist of 48-mer: 5’-GTAATACGACTCACTATAGG GCACGCGTGGTCGACGGCCCGGGCTGGT-3’ and 8-mer with the 3
end capped by an amino residue: 5’-ACCAGCCC-NH2-3). Primer designed by using compound SSR (AC)10; (TC)6(AC)5 
or (AC)6(AG)5 and an adaptor primer AP2 and nested PCR using AP1 primers. The PCR product integrated into the 
plasmid PGEMT Easy Vector System and competence cell Escherichia coli (strain DH5α) and sequence. Eight sequence 
from sample #G17 with SSR compound (AC)10, (TC)6(AG)5 and (AC)6(AG)5 produced two primer pairs. The primer pairs 
from sequence positive clone 4 of SSR compound (TC)6(AC)5 were forward primer: 5’-GGCCGTT AAAGTAGCTCAAGAA
3’ and reverse primer:5’-CCGCATAGCATCAGTATCTG TC-3’, while primer pairs from sequence positive clone 10 of SSR 
compound (AC)6(AG)5 were forward primer: 5’-GTGTTTCCATTTGTTACGCGCT-3’ and reverse primer: 
5’TAATGCCGTTGGGCAGTGA-3’.