Assessment of the antiradical and antioxidant actions of leaf, perilla seed and stalk take out

Abstract

The antioxidant properties of perilla seed, leaf and stalk extracts were evaluated by 2,2-diphenyl-1
picrylhydrazyl (DPPH) and superoxide radicals scavenging activities, reducing power and metal 
chelating ability. The DPPH radical scavenging activity of perilla leaf extract was 57.5%, a value which 
was higher than scavenging activity of other perilla extracts at 15.0 μg/ml. Based on superoxide radical 
scavenging effect, the perilla leaf extract was the most effective (64.2%) one followed by perilla seed 
extract (55.1%) and perilla stalk extract (20.1%) at 10.0 μg/ml. Reducing powers of perilla seed, leaf and 
stalk extracts were excellent. Chelating effects of perilla seed, leaf and stalk extracts were increased 
with the increasing concentration. The data shows that perilla leaf extract has effective antioxidant 
functions, especially in DPPH and superoxide radicals scavenging activities and reducing power. The 
results indicate that the 50% aqueous-methanol extracts of perilla leaf can be used as a new functional 
food and pharmaceutical agent.