Six novel PCR-RFLP loci in milk quality candidate genes in Bubalus bubalis

Abstract

Prolactin receptor (PRLR) and Peroxisome Proliferator Receptor Gamma Coactivator 1 Alpha (PPARGC1A) are 
candidate genes associated with milk protein and fat yield in dairy cattle. These genes were sequence 
characterized to identify exonic SNPs in water buffalo (Bubalus bubalis). Six novel buffalo specific SNPs were 
identified and novel PCR-RFLP loci for these SNPs were developed and validated. These loci can effectively be 
used for genotyping PRLR and PPARGC1A genes in recorded buffalo population for association studies with milk protein and fat yield.