Cytotoxic Effects of Zinc Oxide Nanoflakes (ZNO NFS) in Human Muscle Carcinoma
Keywords:
atomic force microscopy (AFM), δ-aminolevulinic acid (ALA),, reactive oxygen species (ROS), human muscle carcinoma (RD cells), Zinc Oxide nanoflakes ZnO NFs.Abstract
Cytotoxicity of bare and conjugation of Tween-80 capsulated nanomaterials e.g. zinc oxide nanoflakes
(ZnO NFs), iron oxide nanoparticles (Fe2O3 NPs) was examined in dark as well as under light exposure in
cell model of immature human muscle carcinoma (RD) via microinjection and free standing drug delivery
system. ZnO NFs were grown on the tip of a capillary and characterized by applying atomic force
microscopy (AFM) technique and the tip was used as pointer to insert chemicals into cell to
visualize/assess the emission of reactive oxygen species (ROS) especially from mitochondria. Bare and
conjugated ZnO NFs with δ-aminolevulinic acid (ALA) were irradiated /excited with UV light after cellular
uptake, reactive oxygen species were generated. We deduct that ROS is damaging mitochondria
resulting in cell necrosis within few minutes. ZnO NFs are not biosafe and have significant toxic effects
for both normal as well as cancer cell especially for rhybdomyosarcoma cell line (RD).
