The phytase from antarctic bacterial isolate, Pseudomonas sp. JPK1 as a potential tool for animal agriculture to reduce manure phosphorus excretion

Abstract

Intracellular phytase activity was found in the Antarctic bacterial isolate, JPK1. Based on 16S rRNA gene 
sequence analysis, the strain was related to Pseudomonas sp. The optimal activity of JPK1 phytase occurred 
at 50C and pH 5.0 to 5.5. The enzyme was highly specific for phytate with little or no other phosphate 
conjugates. Enzyme activity was strongly inhibited by Cu 2+ and Zn2+, and completely inactivated by sodium 
dodecyl sulfate. The enzyme effectively liberated inorganic phosphate from wheat bran, a main feedstuff with 
high phytate content. The enzyme may be a good candidate for use as an environmental-friendly feed 
additive to enhance the nutritive value of phytate and reduce phosphorus pollution.