Milk ring, rose bengal tests and conventional PCR based detection of Brucella abortus infected dairy cattle in Bangladesh
Keywords:
Seroprevalence, molecular detection,, brucellosis, dairy cattle, Bangladesh.Abstract
The objective of this study was to detect dairy cattle infected with Brucella abortus from Jamalpur,
Rangpur districts and Central Cattle Breeding and Dairy Farm (CCBDF), Savar, Dhaka in Bangladesh.
Both milk and serum samples of 510 dairy cattle were initially screened by milk ring test (MRT) and
Rose Bengal Test (RBT). Twelve samples positive in both MRT and RBT were further confirmed by
conventional polymerase chain reaction (PCR). The overall prevalence of brucellosis based on MRT and
RBT was 2.7 and 2.4%, respectively. The prevalence of brucellosis was found to be significantly
(p<0.001) higher in CCBDF than Jamalpur district. Out of 7 MRT and RBT positive samples, 42.9%
samples of CCBDF were PCR positive and none of the five samples of Jamalpur and Rangpur districts
was PCR positive. These results strongly suggest that the use of MRT, RBT and PCR technique could
lead to more reliable diagnosis of brucellosis from dairy cow in Bangladesh.
