Isolation of total DNA from bacteria and yeast

Abstract

Many procedures in molecular biology require the isolation of high quality genomic DNA. This study 
investigated a new method to extract DNA from Gram-negative, Gram-positive bacteria, Mycobacteria 
and yeasts. Guanidine thioisocyanate present in DNAzol is capable of binding DNA to silica particle 
column. Subsequently the silica with adsorbed DNA is washed to remove impurities and the clean DNA 
eluted in appropriate buffer. Results indicated that the new extraction method is simple and 
reproducible. This isolation technique is faster and easier to perform than the other conventional 
extraction methods. Finally the recovered DNA is of high quality and suitable for downstream 
applications.