Harnessing modern biotechnology for tropical tuber crop improvement: Yam (Dioscorea spp.) molecular breeding
Keywords:
Genetic mapping, genome analysis,, molecular breeding, PCR-based markers,, QTLs, resistance genes, yam.Abstract
Yams (Dioscorea spp.) constitute a staple food crop for over 100 million people in the humid and subhumid
tropics. They are polyploid and vegetatively propagated. The Guinea yams, Dioscorea rotundata and D.
cayenensis, are the most important yams in West and Central Africa where they are indigenous, while D. alata
(referred to as water yam) is the most widely distributed species globally. The genetics of yams is least
understood among the major staple food crops due to several biological constraints and research neglect.
Research to unravel the apparent complexity of the yam genome will have far-reaching implications for
genetic improvement of this important tuber crop. Some progress has been made in recent years in
germplasm characterization and the development of molecular markers for genome analysis. A genetic
linkage map based on amplified fragment length polymorphism (AFLP) markers has been constructed for
Guinea and water yams. These linkage maps were used to scan the genome for quantitative trait loci (QTL)
associated with genes conferring resistance to Yam Mosaic Virus (YMV) in D. rotundata and anthracnose
(Colletotrichum gloeosporioides) in D. alata. In addition, candidate random amplified polymorphic DNA
(RAPD) markers associated with major genes controlling resistance to YMV and anthracnose have been
identified that could be used for selection and pyramiding of YMV and anthracnose resistance genes in yam
improvement. Also, molecular markers such as RAPDs, AFLPs, and microsatellites or simple sequence
repeats (SSRs) have been developed for yam genome analysis. An initial c-DNA library has been constructed
in order to develop expressed sequence tags (ESTs) for gene discovery and as a source of additional
molecular markers. This paper will review the advances made, discuss the implications for yam genetic
improvement and germplasm conservation, and outline the direction for future research.
