Purification of acidic protease from the cotyledons of germinating Indian bean ( Dolichos lablab L. var lignosus) seeds
Keywords:
Dolichos lablab, proteolytic enzymes, acidic protease, purification, protein mobilization, germination.Abstract
The positive correlation between the developments of acid, neutral and alkaline proteases
(azocaseinolytic) with protein depletion suggest the involvement of these proteases in the degradation
of proteins in germinating Indian bean. These proteases increased in the early stages of germination
and decreased later. However, the activity of acid proteases was higher throughout the germination
period compared with the activities of neutral and alkaline proteases. The acid protease from the
cotyledons of 4-day old germinating Indian bean seedlings was purified to 152 folds by a five step
procedure comprising - crude extract from cotyledons, (NH 4)2SO4 fractionation, DEAE-cellulose, CM
cellulose and finally casein-agarose affinity chromatography. The molecular mass of acidic protease is
32 kDa.
