Effects of Cobalt Chloride Stress on Physiological, Biochemical, and Enzymatic Parameters and Protein Profile in Cyamopsis tetragonoloba

Abstract

We investigated the changes in the morphology, biochemical, antioxidant enzyme activity and protein profiles in 
Cyamopsis tetragonoliba (L). The crop was treated with different concentrations of CoCl2 (25, 50, 75, 100, 125 µM-1) and 
the changes in growth, protectively induced oxidative stress in relation to metal stress after 14 days old plant were 
examined. In this study, the lower concentration of 25 µM cobalt chloride has increased the plant productivity than the 
other concentrations tested. Chlorophyll and carotenoid pigments decreased significantly in the stressed leaves for 
their adaptation in stressed condition. The two isozymes CAT 1 and CAT 2 activity were detected from the extract of 
treated and stress leaves were subjected to polyacrylamide gel electrophoresis. Most prominent peroxidase enzymes 
were induced by accumulation CoCl2 and possibly resulting in improved effectiveness of the enzyme at high 
concentration of stressed leaves. The protein profile of cluster bean (Cyamopsis tetragonoliba L.) strongly influenced 
by severe heavy metal stress (100 µM and 125 µM). It was analyzed through SDS-PAGE and found four new polypeptides, 
hence it was concluded that more number of new proteins (190 kDa, 54 kDa, 38 kDa and 33 kDa) were synthesized in 
stress treated plants and it was highly intensive and probably constitutes stress tolerant proteins.