Regeneration of germlings and seedlings development from cauline leaves of Sargassum thunbergii

Abstract

A method of producing artificial Sargassum thunbergii seedlings is urgently desired to meet the increasing demand 
of raw materials used for aquaculture and for the sake of environmental protection. This is the first report of attempt 
to study the vegetative propagation of this species using cauline leaves under laboratory conditions. On average, 
45.75% of the excised leaves survived and one leaf could produce several new individuals. Adventitious burgeons 
grew into branches about 2 mm in length after 3 months of culture. The new individuals were cutoff and could be 
used as seedlings for raft culture and seabed restoration after a further culture to elongate enough for hand-planting.