Interaction of polygalacturonase enzymes from Fusarium oxysporum with tomato polygalacturonase inhibiting protein

Abstract

Polygalacturonases (PGs) are important pectolytic enzymes produced by phytopathogenic fungi during 
the process of infection and colonisation of the host plants. In this work to study the inhibiting effect of 
polygalacturonase inhibiting protein (PGIP) on polygalacturonase (PG) enzyme from Fusarium 
oxysporum f.sp lycopersici, (highly virulent isolates), protein extraction was carried out from various 
tissues (stem, leaf and root) of 40, 60 and 80 days old tomato plants (Lycopersicon esculentum cv. FDT 
202). Study of PGIP- PG interaction showed correlation between the plant age and increasing effect of 
inhibition activity of PGIP, with stem PGIP having the most effect. There were some differences in 
protein patterns of induced and non-induced tomato plants by F. oxysporum. Tomato PGIP showed 
different inhibitory activity on PG extracted from different phytopathogenic isolates of F. oxysporum.