A karyological analysis of PUNTIUS CONCHONIUS (Hamilton, 1822) (Pisces, Cyprinidae), a new cytotype from Dal Lake Srinagar Kashmir, J&K, India

Abstract

The karyotypic and cytological characteristics of Rosy barb, PUNTIUS CONCHONIUS (Hamilton, 1822) (cyprinidae) 
were investigated by examining metaphase chromosomes spreads from the anterior kidney following 
Thorgaard and Disney (1990). The fishes were obtained from the local fishermen and transported live to the 
Limnology and Fisheries Laboratory of Centre of Research for Development, University of Kashmir. Ten fish 
were injected intraperitoneally with two doses of phytohemagglutinin (PHA), 4 µg g-1body weight with a 20 h 
interval to induce cell division. After 8 h in 20°C water, the fish were injected intraperitoneally with colchicine 
0.05% @ 0.5 ml/50 g body weight to depress the cell division in the metaphase stage and left for 2 to 3 h before 
sacrificing. Kidney and gill epithelia were used for karyotype analysis. The diploid chromosome number of the 
fish was 2n = 50, consisting of 11 pairs of metacentric, 8 pairs of submetacentric and 6 pairs of telocentric 
chromosomes respectively. Centromeric index, arm ratio and Fundamental Number were determined as 0 to 50, 
1-∞ and 88 respectively. No heteromorphic sex chromosomes were cytologically detected.