Studies on improved Agrobacterium-mediated transformation in two indica rice (Oryza sativa L.)

Abstract

Agrobacterium tumefaciens strain EHA 105 carrying binary vector pCAMBIA 1301 was used for 
transformation in two economically important highly recalcitrant indica rice cultivars HKR-46 and HKR- 126. 
High concentrations of acetosyringone in the Agrobacterium culture and co-cultivation medium proved to be 
indispensable for successful transformation. Embryogenic scutellar calli were used for transformation 
studies. Binary vector pCAMBIA 1301 have been proved efficient for transformation. The percent transient 
GUS expression found to be higher in cutivar HKR-126 (44.4%) as compared to HKR- 46 (28.9%). The percent 
recovery of hygromycin resistant calli after 4-6 weeks on selection medium was maximum in HKR-126 
(52.6%).