Agrobacterium-induced hypersensitive necrotic reaction in plant cells: a resistance response against Agrobacterium-mediated DNA transfer
Keywords:
Agrobacterium; hypersensitive reaction;, necrosis; signal transduction; oxidative burst;Abstract
High necrosis and poor survival rate of target plant tissues are some of the major factors that affect the
efficiency of Agrobacterium-mediated T-DNA transfer into plant cells. These factors may be the result
of, or linked to, hypersensitive defense reaction in plants to Agrobacterium infection, which may
involve the recognition of specific signals from the Agrobacterium that triggers the burst of reactive
oxygen species at the infection site. Evidences of Agrobacterium-induced necrosis in target plant
tissues and its link to reactive oxygen species are presented. Application of antioxidants, addition of
acetosyringone and optimization of pre-culture conditions suppress the Agrobacterium-induced
hypersensitive necrotic response in target plant tissues, thereby enhancing stable transformation.
