Employment of a new strategy for identification of PRUNUS MUME cultivars using random amplified polymorphic deoxyribonucleic acid (RAPD) markers
Keywords:
Prunus mume, new approach,, random amplified polymorphic deoxyribonucleic acid (RAPD, fingerprints, cultivar identification diagram (CID).Abstract
In this study, we applied a new approach of using random amplified polymorphic deoxyribonucleic acid (RAPD)
fingerprints to distinguish 64 fruiting mei cultivars based on optimization of RAPD through choosing 11 nt primers and
strict screening polymerase chain reaction (PCR) annealing temperature. Results show that this new approach could
clearly utilize and record the fingerprints generated from various primers in cultivar identification and a cultivar
identification diagram (CID) readily constructed. The CID can make mei cultivar identification efficient just like a
periodic table of elements, providing the information needed to separate groups of cultivars as desired. The
workability and efficiency of the method were also well verified. To our best knowledge, this new strategy is the most
efficient and workable in indentifying plant varieties using deoxyribonucleic acid (DNA) markers, which can be of great
help in plant cultivar identification for protection of cultivar rights and for early identification of seedlings in the
nursery industry.
